Journal: Molecular medicine reports

Article Title: MicroRNA-381 inhibits cell proliferation and invasion in endometrial carcinoma by targeting the IGF-1R.

doi: 10.3892/mmr.2017.8288

Figure Lengend Snippet: Figure 1. Suppressed expression of miR‑381 in EC tissues and cell lines. (A) RT‑qPCR analysis of miR‑381 expression in 45 paired EC tissues and corresponding adjacent normal endometrial tissues. *P<0.05 compared with adjacent normal endometrial tissues. (B) miR‑381 expression levels in EC cell lines (HEC‑1B, HEC‑59, AN3CA and KLE) as detected by RT‑qPCR. *P<0.05 compared with adjacent normal endometrial tissues. EC, endome trial carcinoma.

Article Snippet: Human EC cell lines, HEC-1B, HEC-59, AN3CA and KLE, were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing

Journal: Oncology reports

Article Title: Targeting CDK9: A novel biomarker in the treatment of endometrial cancer.

doi: 10.3892/or.2020.7746

Figure Lengend Snippet: Figure 3. CDK9 expression in endometrial cancer cell lines. (A) Expression levels of CDK9 in endometrial cancer cell lines (AN3CA, ARK‑2, HEC‑1A, HEC‑1B, lshikawa, RL95‑2 and SPAC1S) as determined by western blotting. (B) Relative expression of CDK9 and α‑tubulin in the endometrial cancer cell lines. CDK9, cyclin‑dependent kinase 9.

Article Snippet: AN3CA was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany).

Techniques: Expressing, Western Blot

Journal: Oncology reports

Article Title: Targeting CDK9: A novel biomarker in the treatment of endometrial cancer.

doi: 10.3892/or.2020.7746

Figure Lengend Snippet: Figure 5. CDK9 inhibitor reduces endometrial cancer cell proliferation by suppressing transcription elongation and inducing apoptosis in endometrial cancer cells. (A and B) Relative cell viability of AN3CA and SPAC1S cells after exposure to different concentrations of the CDK9 inhibitor LDC067 for 5 days. **P<0.01 compared with the lowest concentration group (1x10‑3 µM). (C and D) Expression levels of CDK9 and related signaling pathway proteins involved in transcription and apoptosis after treatment with LDC067 in cells by western blot analysis. CDK9, cyclin‑dependent kinase 9; Mcl‑1, myeloid cell leukemia‑1; Bax, proapoptotic protein BCL2 associated X, apoptosis regulator; PARP, poly(ADP‑ribose) polymerase.

Article Snippet: AN3CA was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany).

Techniques: Concentration Assay, Expressing, Western Blot

Journal: Oncology reports

Article Title: Targeting CDK9: A novel biomarker in the treatment of endometrial cancer.

doi: 10.3892/or.2020.7746

Figure Lengend Snippet: Figure 4. CDK9 knockdown by siRNA transfection suppresses endometrial cancer cell proliferation. (A and B) MTT assay revealed significant dose‑dependent inhibition of cell proliferation after CDK9 siRNA treatment. **P<0.01 compared with the cell only control group. (C and D) Expression levels of CDK9 and related signaling pathway proteins involved in transcription and apoptosis after transfection of CDK9 siRNA and nonspecific siRNA (NC siRNA) in AN3CA and SPAC1S cell lines by western blot analysis. CDK9, cyclin‑dependent kinase 9; Mcl‑1, myeloid cell leukemia‑1; Bax, proapoptotic protein BCL2 associated X, apoptosis regulator.

Article Snippet: AN3CA was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany).

Techniques: Knockdown, Transfection, MTT Assay, Inhibition, Control, Expressing, Western Blot

Journal: Oncology reports

Article Title: Targeting CDK9: A novel biomarker in the treatment of endometrial cancer.

doi: 10.3892/or.2020.7746

Figure Lengend Snippet: Figure 7. Inhibition of CDK9 reduces endometrial cancer cell migration. (A and B) Representative images of AN3CA and SPAC1S cell migration after CDK9 inhibitor LDC067 treatment for 0, 24, and 48 h. (C and D) Quantification of cell migration distance of AN3CA and SPAC1S cells after LDC067 treatment. **P<0.01 compared with the Cell only group. CDK9, cyclin‑dependent kinase 9.

Article Snippet: AN3CA was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany).

Techniques: Inhibition, Migration

Journal: Oncology reports

Article Title: Targeting CDK9: A novel biomarker in the treatment of endometrial cancer.

doi: 10.3892/or.2020.7746

Figure Lengend Snippet: Figure 6. Inhibition of CDK9 suppresses endometrial cancer cell colony formation. (A) Representative images of endometrial cancer cell colony formation after incubation with different concentrations of LDC067 (0, 2.5, 5.0, and 10 µM) for 14 days. (B and C) Quantification of clonogenicity formation of AN3CA (B) and SPAC1S (C) cells after LDC067 treatment. **P<0.01 compared with the Cell only group. CDK9, cyclin‑dependent kinase 9.

Article Snippet: AN3CA was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany).

Techniques: Inhibition, Incubation

Journal: Molecular Medicine Reports

Article Title: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

doi: 10.3892/mmr.2024.13308

Figure Lengend Snippet: ADIPOR1 is associated with the endometrial receptivity. (A) ADIPOR1 mRNA expression during the proliferative and secretory phases in endometrial tissues obtained from fertile patients, and during the secretory phase in endometrial tissues obtained from infertile patients. (B) Spheroid attachment to receptive RL95-2 cells and non-receptive AN3CA cells. (C) E-cadherin expression in RL95-2 and AN3CA cells. (D) ADIPOR1 mRNA expression in RL95-2 and AN3CA cells. (E) Analysis of ADIPOR1 expression in RL95-2 and AN3CA cells. Data are presented as the mean ± standard error of the mean of values from three independent experiments (n=3). *P<0.05, **P<0.01. NS, not significant; ADIPOR1, adiponectin receptor 1.

Article Snippet: Human endometrial cell lines (RL95-2 and AN3CA) were acquired from the American Type Culture Collection.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

doi: 10.3892/mmr.2024.13308

Figure Lengend Snippet: ADIPOR1 regulates E-cadherin mRNA and protein expression in endometrial epithelial cells. (A) Effect of ADIPOR1 knockdown on corresponding mRNA expression in RL95-2 cells. (B) Effect of ADIPOR1 knockdown on corresponding protein expression in RL95-2 cells. (C) Rate of spheroid attachment to ADIPOR1-knockdown RL95-2 cells. (D) Effect of ADIPOR1 knockdown on downregulation of -E-cadherin in RL95-2 cells. (E) Effect of ADIPOR1 knockdown on downregulation of E-cadherin in RL95-2 cells. Data are presented as the mean ± standard error of the mean of values from three independent experiments (n=3). *P<0.05, **P<0.01. ADIPOR1, adiponectin receptor 1; sh, short hairpin.

Article Snippet: Human endometrial cell lines (RL95-2 and AN3CA) were acquired from the American Type Culture Collection.

Techniques: Expressing, Knockdown

Journal: Molecular Medicine Reports

Article Title: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

doi: 10.3892/mmr.2024.13308

Figure Lengend Snippet: ADIPOR1 regulates AMPK activity in endometrial epithelial cells. (A) p-AMPK/AMPK protein expression in RL95-2 and AN3CA cells. (B) p-AMPK/AMPK protein expression ADIPOR1-knockdown RL95-2 cells. Data are presented as the mean ± standard error of the mean of values from three independent experiments (n=3). **P<0.01. ADIPOR1, adiponectin receptor 1; AMPK, AMP-activated protein kinase; p-, phosphorylated; sh, short hairpin.

Article Snippet: Human endometrial cell lines (RL95-2 and AN3CA) were acquired from the American Type Culture Collection.

Techniques: Activity Assay, Expressing, Knockdown

Journal: Molecular Medicine Reports

Article Title: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

doi: 10.3892/mmr.2024.13308

Figure Lengend Snippet: AMPK inhibition by dorsomorphin attenuates endometrial receptivity in receptive RL95-2 cells. (A) Analysis of AMPK inhibition by dorsomorphin via immunoblotting. (B) Effect of dorsomorphin on the spheroid-attachment rate in RL95-2 cells. (C) E-cadherin mRNA expression in RL95-2 cells treated with dorsomorphin. (D) E-cadherin expression in RL95-2 cells treated with dorsomorphin. Data are presented as the mean ± standard error of the mean of values from three independent experiments (n=3). *P<0.05, **P<0.01. NS, not significant; AMPK, AMP-activated protein kinase.

Article Snippet: Human endometrial cell lines (RL95-2 and AN3CA) were acquired from the American Type Culture Collection.

Techniques: Inhibition, Western Blot, Expressing

Journal: Molecular Medicine Reports

Article Title: Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway

doi: 10.3892/mmr.2024.13308

Figure Lengend Snippet: AMPK activation by AICAR induces endometrial receptivity in non-receptive AN3CA cells. (A) AMPK activation by AICAR assessed via immunoblot assay. (B) Effect of AICAR on the spheroid-attachment rate in AN3CA cells. (C) E-cadherin mRNA expression in AN3CA cells treated with AICAR. (D) E-cadherin expression in AN3CA cells treated with AICAR. Data are presented as the mean ± standard error of the mean of values from three independent experiments (n=3). *P<0.05, **P<0.01. NS, not significant; AMPK, AMP-activated protein kinase; AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide; p-, phosphorylated.

Article Snippet: Human endometrial cell lines (RL95-2 and AN3CA) were acquired from the American Type Culture Collection.

Techniques: Activation Assay, Western Blot, Expressing

Journal: Heliyon

Article Title: Uncovering a novel DNA repair-related radiosensitivity model for evaluation of radiotherapy susceptibility in uterine corpus endometrial cancer

doi: 10.1016/j.heliyon.2024.e29401

Figure Lengend Snippet: Validation of the radiosensitivity genes in vitro experiment. (A) The expression levels of 4 radiosensitivity genes between the RS and RR groups in the boxplot. (B) The correlation analysis of 4 radiosensitivity genes. (C) The proliferative capacity of AN3CA and AN3CA/IR cells after radiation exposure was evaluated using the CCK8 assay. (D) The qRT-PCR was utilized to assess the expression levels of WRN and APEX1 in AN3CA and AN3CA/IR cells. **p < 0.01, ***p < 0.001.

Article Snippet: The human endometrial cancer cell line AN3CA was purchase from the China Center for Type Culture Collection (CCTCC, Wuhan, China).

Techniques: Biomarker Discovery, In Vitro, Expressing, CCK-8 Assay, Quantitative RT-PCR